Strain Typing Methods and Molecular Epidemiology ofPneumocystisPneumonia
نویسندگان
چکیده
منابع مشابه
Molecular Methods for Bacterial Strain Typing
ABSTRACT Typing of bacteria is an important part of epidemiological studies on nosocomial infections. Bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. In many cases, molecular techniques have replaced phenotypic typing methods. Currently, a wide r...
متن کاملStrain Typing Methods and Molecular Epidemiology of Pneumocystis Pneumonia
Pneumocystis pneumonia (PCP) caused by the opportunistic fungal agent Pneumocystis jirovecii (formerly P. carinii) continues to cause illness and death in HIV-infected patients. In the absence of a culture system to isolate and maintain live organisms, efforts to type and characterize the organism have relied on polymerase chain reaction-based approaches. Studies using these methods have improv...
متن کاملmolecular methods for bacterial strain typing
abstract typing of bacteria is an important part of epidemiological studies on nosocomial infections. bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. in many cases, molecular techniques have replaced phenotypic typing methods. currently, a wide range of bacterial typing techniques is...
متن کاملApplication of whole-genome sequencing for bacterial strain typing in molecular epidemiology.
Nosocomial infections pose a significant threat to patient health; however, the gold standard laboratory method for determining bacterial relatedness (pulsed-field gel electrophoresis [PFGE]) remains essentially unchanged 20 years after its introduction. Here, we explored bacterial whole-genome sequencing (WGS) as an alternative approach for molecular strain typing. We compared WGS to PFGE for ...
متن کاملMethicillin-resistant Staphylococcus aureus in Spain: molecular epidemiology and utility of different typing methods.
In a point-prevalence study performed in 145 Spanish hospitals in 2006, we collected 463 isolates of Staphylococcus aureus in a single day. Of these, 135 (29.2%) were methicillin (meticillin)-resistant S. aureus (MRSA) isolates. Susceptibility testing was performed by a microdilution method, and mecA was detected by PCR. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE) afte...
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ژورنال
عنوان ژورنال: Emerging Infectious Diseases
سال: 2004
ISSN: 1080-6040,1080-6059
DOI: 10.3201/eid1010.030981